Abstract
Ayurveda has given most importance to the Vata amongst the three Doshas. Benign Prostate hypertrophy is one of the most important diseases of old age in which there is vitiation of Vata mainly. This disease is characterized by frequency of micturition, urgency of micturition etc. In Ayurveda classics, it is stated that Basti is the best treatment and Dashamoola is one of best combination of drugs for alleviation of vitiated Vata. The present study deals with the standardization of contents of Dashamooladi Basti through the pharmaceutical standards. Pharmaceutical analysis showed that Ash value and Specific gravity of Madhu is 0.23%w/w and 1.30, Acid value and saponification value of Dashamoola taila is 3.64 and 193.95, pH and total solid content of Basti is 4.89 and 36.93%, refractive index of Dashamoola taila is 1.47. On the basis of observations and experimental results, the study may be used as standard protocol in the further quality control researches.

Keywords: Dashamooladi Basti, Benign Prostate hypertrophy, Pharmaceutical.

Introduction:
Benign Prostate Hypertrophy is the disease which very commonly occurs amongst the old aged male persons. This disease occurs mainly due to vitiation of Vata. This disease is characterized by frequency of micturition, urgency of micturition etc. In Ayurveda classics, it is stated that Basti is the best treatment and Dashamoola is one of best combination of drugs for alleviation of vitiated Vata. Dashamooladi Basti contains Madhu (Honey), Dashamoola Kwatha (Decoction), Dashamoola taila (oil), Saindhava (Sodii chloridum). Among the contents of Dashamoola, most of the contents have Madhura rasa and Ushna veerya and Saindhava have Lavana rasa and Ushna veerya which plays an important role in alleviation of vitiated Vata. Present study has been selected for Pharmaceutical standardization of Dashamooladi Basti.

Materials and Method:

Collection and authentification of raw drugs:
Roots of all the contents of Dashamoola, Saindhava, Madhu were collected from periphery of Mumbai (Maharashtra). The ingredients with botanical name and family are mentioned in Table.1.

Method of Preparation of Dashamooladi Basti:
All the pre authenticated raw drugs (Table 1) were taken for the preparation. First dashmoola siddha taila and Dashmoola kwatha prepared according to Sharangadhara samhita. While preparing Dashamooladi basti Drug mix in proper sequence, first Madhu and Saindhava mixed. Then siddha taila added to it. Finally Kwatha added to it in proper proportion.

Pharmaceutical analysis:
Dashamooladi basti was analyzed using various standard physicochemical parameters such as loss on drying; acid value, saponification value, specific gravity, refractive index and pH value were carried out as per procedures.

Observation and Discussion:
This study is an effort towards pharmaceutical standardization of contents of Dashamooladi basti. In Madhu (Honey), Ash value was found o.23%w/w and specific gravity was found 1.30. In Saindhava, loss on drying was found 0.09%w/w and pH was found 6.31. In Dashamoola kwatha, pH was found 4.93 and total solid content was found 1.81%. In Dashamoola taila, Acid value was found 3.64 while saponification value was found 193.95, refractive index was found 1.47. In the final product i.e. Dashamooladi basti, pH was found 4.89 while total solid content was found 36.93%.

Conclusion:
In preliminary pharmaceutical analysis, ash value, specific gravity, pH, loss on drying, refractive index, acid value, saponification value were assessed. The results of this study may be used as the reference standard in further research undertakings of its kind.

References:
1. Shodhala, Gadanigraha, edited by Gangasahay Pandey, Varanasi: Chaukhamba Sanskrit Sansthan; Vol.II, Edition 2005. p. 551
2. Anonymous. The Ayurvedic Pharmacopoeia of India. Part 1, Vol. 1, 2, 4, 5 1st ed. New Delhi: Ministry of Health and Family welfare, Department of AYUSH Government of India; 2001.
3. Gokhale S.B, Kokate C.K. Practical Pharmacognocy, Pune: PragatiBooks Pvt; 2008 p. 13.
4. Kakate C K, Practical Pharmacognocy, 4 thed, Delhi; VallabhPrakashan; 2005. P. 7-9, 22-3.
5. Showdon D W, Janckson BP. Atlas of Microscopy of Medicinal Plants, Clinary herbs and Spices. 1sted.
New Delhi: CBS Publishers and Distributors; 2005. p. 14-7.
6. Platel, K., Srinivas, K., Nahrung 2000, 44, 42-46.
7. Hussain A, Naz S, Nazir H, Shinwari ZK. Tissue culture of Black pepper (Piper nigrum L.) in Pakistan.Pak. J. Bot 2011; 43:1069-1078.

8. Woolf 1999; Bakowski and Michalik 1986; Halva and Pukka 1987; Paakkonen et al.1989; Guenther 1950.