These days, the final barrier is the origin of life: The scientist goes about it in time honored fashion: the tree of life appears on a page, the seed is invisible.
The dilemma is this: whereas there are pre-life forms, even amino acids in chains, and life forms, marked by the ability to multiply, the key to life is unresolved.
Science logically assumes life is based in chemistry, but it could be said equally chemistry is a manifestation of life, in every entity that manifests a form.
Forms affect each other with a specific contextual effect, and work towards synthesis of identities made manifest as have their origin in context, including those of life forms.
Thus life forms are no different to non-life in terms of being contextual identities, and indicate rather the life that is the context in which all forms are realized as concepts.